Phytochemicals and antibacterial activity of aerial parts and roots of pimpinella candolleana wight & arnott

Infections still cause about one-third of all deaths worldwide and are the leading cause of death, mainly because of disease in developing countries. Pimpinella candolleana Wight & Arnott belonging to the family Apiaceae and Shan kokkaya in Myanmar was collected from Pindaya Township, Southern Shan State in 2015. The phytochemical, physico-chemical properties of crude aerial parts and root powdered were determined by Raaman (2006) and WHO (2011). The elemental compositions of both crude powders were analyzed by the Wavelength Dispersive X-ray Fluorescence. The antibacterial activity of the different concentrations of five extracts of the aerial parts and roots were assayed by agar disc diffusion method. In preliminary phytochemical investigation, flavonoids, glycosides, polyphenol, steroids, reducing sugar, amino acids and tannins were observed in root powder but reducing sugar, amino acids and carbohydrates are absent in aerial parts. In the physico-chemical analysis, the total ash content, acid insoluble and water soluble ash were presented and the both samples were more soluble in polar solvents. In elemental analysis, potassium, calcium, iron, phosphorus, chlorine, sulfur, magnesium, silicon, zinc and manganese were detected in both samples. The aqueous methanolic and pet-ether extracts of both samples had moderate activity on Escherichia coli and Staphylococcus aureus at 20 mg/disc concentration and pet-ether extract showed significant dose response relationship on Staphylococcus aureus. Therefore, this plant extracts possess various effective secondary metabolites, beneficial elements and antibacterial activity.