Pharmaceutical analysis plays a vital role in the Quality Assurance and Quality control of bulk drugs. A rapid and precise reverse phase high performance liquid chromatographic method has been developed for the validated of benserazide and levodopa, in its pure form as well as in tablet dosage form. Chromatography was carried out on an Altima C18 (4.6 x 150mm, 5µm) column using a mixture of methanol: TEA buffer pH 4.5: acetonitrile (50:25:25) as the mobile phase at a flow rate of 1.0ml/min, the detection was carried out at 225nm. The retention time of the benserazide and levodopa was 2.102, 3.537±0.02min respectively. The method produces linear responses in the concentration range of 5-25mg/ml of benserazide and 12.5-62.5mg/ml of levodopa. The method precision for the determination of assay was below 2.0 %RSD. The method is useful in the quality control of bulk and pharmaceutical formulations. The validated method was successfully applied to quantify levodopa and benserazide simultaneously in a pharmaceutical formulation. The method was found to be precise, sensitive and accurate for the simultaneous determination levodopa and benserazide in bulk and pharmaceutical formulations.
